Mark 4as the current version of the lsm 510 is charac. The system provides high resolution images of fluorescent molecules with improved signal noise ratio compared to wide field fluorescence microscopy. The objectives currently installed vary from 5x to 63x magnifications. Get free microscope brochures, software updates or hotfixes. Hence, start the plugin from the plugin menu while no image is open. The lsm 5 lets you implement added lsm software functionality and completely automated problem solutions controlled by macros including everything from lsm setting control to image processing and documentation. Theres also the useful zeiss confocal lms 510 poster that is well word a read. Due to the large number of import and export filters e. The lsm 510 meta is a very powerful and modular tool that is able to meet your research demands. As a result you acquire optical sections with high contrast and high resolution in x, y and z. For sale is a zeiss lsm510 meta nlo ready laser scanning confocal microscope system. You will need to put down your name on the reservation system. Advantages disadvantages slower image acquisition single track use for single, double and.
Lsm 510 notes on device safety lsm 510 meta general regulations carl zeiss b 450008 e 1002 1 notes on device safety 1. The achievable scan speed is from a range of 5 fps to 70 fps for 512x 512 pixel resolution. In addition, mark 3 offered a broader choice of lasers, including modern diode versions e. Selecting an objective and focusing the microscope. Training guide for zeiss lsm 510 baylor college of medicine. Konfokales laserscanningmikroskop zeiss lsm 510 meta.
Zeiss lsm510 meta confocal imaging system wellcome. Lsm 510 meta laser scanning mikroskop fcam frankfurt. Clicking on this button activates the complete lsm hardware online. This microscope has the multichannel meta detector for spectral imaging. Important zeiss downloads for microscopy and imaging. It retrieves all of the image capture and calibration information stored in zeiss. Files generated by the 510 lsm software can be loaded by the zen. A traditional point scanning confocal laser scanning microscope clsm with excitation from 405 to 633 and multichannel imaging. Zeiss lsm510 axio meta laser scanning confocal microscope system. The lsm 510 and lsm 510 meta laser scanning microscope. Before looking at the sample in the microscope press the vis button. Finally, the zeiss manual describing the lsm 510 meta confocal system and software for obtaining and analysing images, and the manual which describes the use of the confocor system for fcs use, are both also available for download. Zeiss lsm 510 meta inverted confocal manual adapted from the. Zeiss microscope lsm 510 meta confocal with axiovert 200.
Zeiss lsm 510 meta confocal microscope operating instructions starting the system. You can even try out demo software for light and laser scanning microscopes free of charge. Solveig hehl, tasc carl zeiss jena lsm 510 meta efguided tour april 2002 getting started 1 start the software 3 select laser switch on required laser lines argon laser power should be 60 % 4 select micro choose objective lens from pull down menu select appropriate filterset for fluorescence e. Zeiss lsm 510 meta microscope harvard neurodiscovery center. Lsm 510 upright confocal with meta detector and mp laser isu. It is hooked to an upright fully motorized axio imager z1 microscope. Systempc on components on computer tower on login with your user name and password to biotech domain doubleclick start zen start system initialize hardware e.
It is the hbo 50 box sitting on the desk with the monitor 2. Zeiss 510 meta w zen confocal microscope handbook revised 01152010 startup hbohg lamp on below table little box beside the keyboard. Zeiss 510 meta pennington biomedical research center. The zeiss lsm 510 microscope has three visible wavelength lasers, a coherent chameleon multiphoton excitation mpe laser tunable from 720nm 930nm a meta spectral emission detector and 4 nlos external detectors. The zeiss simplified lms 510 guide and our inhouse. Starting the zeiss lsm 510 microscope, software and laser selecting an objective and focusing the microscope configuring the laser scanning and detection for confocal image acquisition acquiring a z and time series data storage descriptions also include the lsm 510 meta. Confocal microscopes zeiss lsm 800 laser scanning confocal microscope az w5 the zeiss lsm 510 meta microscope located in az w5 was recently upgraded to an lsm 800. The lsm 510 meta laser scanning confocal microscope from zeiss is a spectral imaging.
Single and batch file export from the lsm 510 in other file formats tif, bmp, avi, quicktime, and more. Lsm 510 and lsm 510 meta laser scanning microscopes brief operating manual release 4. Zeiss axiovert 200 microscope lsm 510 meta confocal laser scanning microscope laser modul uvir comes with 6 laser lines 405, 458, 488, 514, 543, 633 motorized stage and controller. In the operating manual, read the chapter safety instructions carefully before. The combination of the lsm scanhead, axioskop 2 fs mot, coherent ti. The zeiss lsm 510 meta is the workhorse confocal microscope at the bosch institute advanced microscopy facility. In the meantime the previous software is available on the system. Flip the two toggle switches on top of the cpu from o to. Lsm 510 upright confocal with meta detector and mp laser. Under this tab you can turn on and off light to the microscope. Microscopes every lsm 510 meta is based on one of the highperformance research microscopes from zeiss. Konfokales laserscanningmikroskop zeiss lsm 510 meta chair.
Zeiss lsm 510 meta guided tour 7 starting the zeiss lsm 510 microscope, software and laser selecting an objective and focusing the microscope selecting an objective and focusing the microscope configuring the laser scanning and detection for confocal image acquisition acquiring a z and time series data storage. Zeiss lsm 510 meta multiphoton microscope cell imaging core. The lsm 510 switchboard window appears on the screen fig. Double click the lsm 510 icon on the desktop of windows to start the lsm 5 software program. Free lsm image browser comes with many functions from the zeiss lsm software package. Equipment and resources section submenu equipment and resources. The sample was imaged with a zeiss lsm meta 510 using 8 evenly spaced spectral emission channels from 508 to 657 nm width 21. Zen 2009 icon double click on this icon to start the software. Depending on your specific applications, you can choose between the following instruments.
The lsm 150 meta features improved sensitivity, automatic component extraction through intelligent software, and increased excitation flexibility for a broader range of fluorescent dyes and proteins. Zeiss lsm 510meta confocal laser scanning microscope. The new pc with new zen software is being fixed by zeiss. A zeiss lsm 510 meta operating manual is available on the book shelf to read for more detailed discussions of the microscope operation. Zeiss lsm 510 meta user manual hege avsnes dale, molecular imaging center, uib. We have 3 zeiss lsm 510 meta microscopes, one equipped with a confocor2 for fluorescence correlation spectroscopy fcs and another with a femtosecond laser for multiphoton microscopy. Confocal laser scanning microscopes scan samples sequentially point by point, or multiple points at once. Plus theres downloadable pdfs for autofluorescence information, gfp and crosstalk spectral bleedthrough, spectral separation using the 510 meta head, and the related emission fingerprinting lsm software. Carl zeiss macro description lsm 510 lsm 510 meta 1 may 2005 macro solutions.
Also available are the revised edition of handbook of biological confocal microscopy edited by james b. Please be patient with this instrument while we attempt to alleviate bugs as they are discovered. Lsm 510 software must be running to use the axiovert 200 microscopelogon to windows with. Confocal microscope usage manual zeiss lsm 510 meta date. Overview of confocal laser scanning microscopes zeiss. Turn the black knob on the laser box onequarter turn from off to on. Zeiss lsm 510 meta fakultat universitat greifswald. Confocal microscope usage manual zeiss lsm 510 meta. Starting the zeiss lsm 510 microscope, software and laser.
Zeiss lsm image browser is a software program that lets you manage pictures that have been generated with the help of carl zeiss laser scanning system lsm 510 and lsm 5 pascal. Three lasers capable of providing a total of 7 excitation wavelengths 405 uv range dyes, 458, 477, 488, 514, tunable argon for blue dyes, 543 for red dyes, and 633nm for far red dyes. Click on the scan new images button in the lsm 510 switchboard window. Zeiss 510 operating procedures pdf format zeiss brochure for lsm 510 nlo. The chameleon provides widely tunable femtosecond pulses with more than 1w of average power. Olympus lext ols4000 laser measuring microscope tested w software. Please contact carl zeiss if you intend to use a laser type with a wavelength. Turn on the mercury arc lamp if you intend to use it. This spectral confocal microscope is based on an inverted zeiss axioobserver microscope. The lsm 510 lsm 510 meta lsm 510 nlo laser scanning microscope, including its original accessories and compatible accessories from other manufacturers, may only be used for the purposes and microscopy techniques described in this manual intended use.
Used zeiss lsm510 meta nlo ready microscope for sale. Zeiss lsm 510 meta inverted laser scanning confocal microscope with multiphoton nlo laser this confocal is on an inverted motorized zeiss axiovert 200 microscope. Zeiss 510 confocal users, with the implementation of this new instrument, several software hardware bugs have already been identified. Zeiss lsm 510 meta guided tour 20 choosing the configuration multi track use for double or triple labelling sequential scanning, line by line or frame by frame when one track is active, only one detector and one laser is switched on. You can minimize it if you like, but do not close it. Guide to confocal microscopy on the zeiss lsm 510 meta nlo. Turn on the mercury lamp if required and sign in the log book, recording lamp hours. Image browser is a free strippeddown version of the lsm 510 software. The new system includes solid state lasers at 405 nm, 488 nm, 561 nm and 642 nm. The lsm 510 meta enables you to easily perform quantitative colocalization analyses with reliability and precision. Laser scanning confocal microscope lscm with meta detector module which can scan across a range of wavelengths to allow digital separation of overlapping spectra such as gfp and yfp. The argon laser has a very specific power routine listed below.
Guided tour for lsm 510 and meta welcome to marlin. It allows multifluorescence images to be collected without compromising resolution and efficiency. Included was intelligent software such as automatic component extraction and improved sensitivity with 1250 v highgain pmts. Lsm 510 introduction to laser scanning microscopy lsm 510 meta performance features of the lsm 510 carl zeiss performance features of the lsm 510 3. The zeiss lsm software provides a wide range of image processing functions, including all standard 2d3d stereo, projection functions, digital processing of voxels and 3d measurement functions and parameters. The lsm 510 switchboard window appears on the screen. Zeiss lsm 510 meta inverted confocal microscope m078a smith features include.
Lsm 510 and lsm 510 meta laser scanning microscopes. It has a number of lasers for excitation of fluorescent dyes and fluorescent proteins. We also have a olympus fv confocal microscope with enclosed chamber for live cell imaging, a leica tcs sp5 ii confocal microscope and a leica am tirf mc that. The lsm 510 meta laser scanning confocal microscope from zeiss is a spectral imaging confocal system. Zeiss lsm 510meta confocal laser scanning microscope wur. Microscope confocal lsm510 meta welcome to the zeiss lsm 510 meta confocal tutorial. Zeiss lsm 510 meta inverted confocal microscope m078a smith. Zeiss lsm 510 meta user manual universitetet i bergen. The microscope is again fully operational, lasers aligned and cleaned. Sa laser and the straightforward software works from scratch.609 270 713 1390 1059 1329 282 1396 531 466 772 753 994 242 1436 67 321 352 1466 528 938 942 1165 1304 768 471 1333 812 720 984 313 161 1325 624 808 800 778 1286 1277 413 370 76 66